Innovative Methodology An efficient system for tissue-specific overexpression of transgenes in podocytes in vivo
نویسندگان
چکیده
Marcus J. Moeller, Abdulsalam Soofi, Silja Sanden, Jürgen Floege, Wilhelm Kriz, and Lawrence B. Holzman Institute for Anatomy and Cell Biology, University of Heidelberg, Heidelberg; Division of Nephrology and Immunology, University Hospital, Rheinisch-Westfalischen Technischen Hochschule, Aachen, Germany; and Department of Internal Medicine, University of Michigan Medical School, Ann Arbor, Michigan
منابع مشابه
An efficient system for tissue-specific overexpression of transgenes in podocytes in vivo.
The utility of promoter fragments isolated from the 5'-flanking region of endogenous mammalian genes to drive transgene expression in vivo is often limited by low expression levels. In this study, a bigenic system was established that allows constitutive overexpression of transgenes in a tissue-specific fashion in transgenic mice in a time- and cost-effective fashion. A modified floxed expressi...
متن کاملPodocyte-specific overexpression of the antioxidant metallothionein reduces diabetic nephropathy.
Podocytes are critical components of the selective filtration barrier of the glomerulus and are susceptible to oxidative damage. For investigation of the role of oxidative stress and podocyte damage in diabetic nephropathy, transgenic mice that overexpress the antioxidant protein metallothionein (MT) specifically in podocytes (Nmt mice) were produced. MT expression was increased six- and 18-fol...
متن کاملروش های ژن درمانی برای ترمیم و نوسازی دستگاه عضلانی اسکلتی
Injuries to the musculoskeletal system are common, debilitating and expensive. In many cases, healing is imperfect, which leads to chronic impairment. Gene transfer might improve repair and regeneration at sites of injury by enabling the local, sustained and potentially regulated expression of therapeutic gene products such products include morphogens, growth factors and anti-inflammatory agent...
متن کاملEstablishment of Protein Delivery Systems Targeting Podocytes
BACKGROUND Podocytes are uniquely structured cells that are critical to the kidney filtration barrier. Their anatomic location on the outer side of the glomerular capillaries expose podocytes to large quantities of both plasma and urinary components and thus are reachable for drug delivery. Recent years have made clear that interference with podocyte-specific disease pathways can modulate glome...
متن کاملmiR-93 regulates Msk2-mediated chromatin remodelling in diabetic nephropathy
How the kidney responds to the metabolic cues from the environment remains a central question in kidney research. This question is particularly relevant to the pathogenesis of diabetic nephropathy (DN) in which evidence suggests that metabolic events in podocytes regulate chromatin structure. Here, we show that miR-93 is a critical metabolic/epigenetic switch in the diabetic milieu linking the ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
دوره شماره
صفحات -
تاریخ انتشار 2005